Value Stream
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From Ideas
to Impact.

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PeopleProcessAIDiscoveryImpact
Methods · for principal investigators and their labs

Your analysis lives in your hands. Methods is a guided talk-through with an agent about exactly how you get from raw data to a result: the export, the spreadsheet, the replicate you drop, the threshold you set by eye. It asks for the details you would never think to write, produces a workflow a second scientist can reproduce, and names the by-hand steps a script could take over. You stay the scientific oversight at every step.

Request early access See how a session works Early cohort: open-science-funded labs, autumn 2026.
Workflow · qPCR relative expression · v1 · draft for sign-off9 steps · 3 flags
Analysis, as stated
  1. 1Export Ct values from the instrument software (version: Not stated) to Excel.
  2. 3Exclude disagreeing replicates. Rule: by eye. Threshold: Not stated
  3. 5ΔΔCt against GAPDH, efficiency assumed 100%. Reference-gene stability: unverified — "I have never checked"
  4. 8Plot fold change in Prism; error bars from technical replicates. Candidate: scripted ΔΔCt with QC rule and biological-replicate stats
Standardization map
Variance points3 judgment calls, 1 with a stated rule
Already has a toolReference-gene stability · geNorm / NormFinder
Needs a scriptReplicate QC + ΔΔCt + stats, one run
Not stated3 values, awaiting you
Your call · sign off before exportevery line traces to what you said
Where we sit

Seven stages carry an idea to impact. Methods works where the data leaves the instrument.

Design, execution and analysis are where a workflow lives in one person's hands and where a by-hand step quietly becomes result-to-result variance. Analysis is where we start; that is the part we capture and, when you are ready, help you automate.

Idea &
hypothesis
Literature
research
Experimental
design
Execution &
data generation
Analysis &
insights
Collaboration &
publication
Translation
& impact
Stages Methods covers today. The rest of the chain is where your protocol goes next.
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What the site covers

The problem

Zero of 193 experiments could be attempted from the published methods alone. The missing part is in someone's hands.

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How it works

A chat, one question at a time, chasing the by-hand steps in your analysis. You sign off before anything leaves.

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Tiers

Establish, Build, Connect. Annual plans per lab, and a named point in each tier where you make the call.

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What stays yours

Ownership follows your institution's policy. No training on your protocols. Wrong is visible, not hidden.

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Who it is for

Labs that have to deposit complete protocols, starting with open-science funders.

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Early access

Bring one analysis you run by hand. Autumn 2026 cohort.

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Science multiplied.
Scientists  ×  Workflows  ×  AI  ×  Impact